RT Journal Article
SR Electronic
T1 Effect of Tween 80 on incubation period of Lactobacillus casei assay of serum folate.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 227
OP 229
DO 10.1136/jcp.31.3.227
VO 31
IS 3
A1 Tennant, G B
YR 1978
UL http://jcp.bmj.com/content/31/3/227.abstract
AB Contamination of folic acid assay medium with oleate led to a lag phase when Lactobacillus casei cultures were grown on folic acid but not when whole serum provided the folate source. This led to high assay values when using 20-hour incubation periods but satisfactory results were obtained after 48 hours' incubation. The addition of Tween 80 to the medium eliminated the lag phase and the assay results were consistent after 13, 24, and 48 hours' incubation.