RT Journal Article
SR Electronic
T1 Production of freeze-dried human antihaemophilic cryoprecipitate.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1091
OP 1093
DO 10.1136/jcp.34.10.1091
VO 34
IS 10
A1 Millgan, G
A1 Graham, R
A1 Hanratty, S
A1 Muir, W
A1 Mitchell, R
YR 1981
UL http://jcp.bmj.com/content/34/10/1091.abstract
AB Concentration of factor VIII from fresh plasma by cryoprecipitation remains the basis for preparation of products used to treat haemophilia A. This paper describes the preparation of a factor VIII concentrate from small plasma pools in transfusion centres with drying facilities. The dried concentrate from one litre of plasma dissolves very well in 50 or 100 ml of distilled water and contains around 500 IU per bottle. The specific activity per mg protein is 0.19 IU and the fibrinogen concentration is half that in frozen cryoprecipitate. This method of drying causes no appreciable loss in the factor VIIIC activity and little denaturation as shown by the factor-VIII-related antigen/factor VIIIC ratio of 1.7.