RT Journal Article
SR Electronic
T1 Imprint cytology in immunocytochemical analysis of oestrogen and progesterone receptors of breast carcinoma.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1043
OP 1045
DO 10.1136/jcp.42.10.1043
VO 42
IS 10
A1 Helin, H J
A1 Isola, J J
A1 Helin, M J
A1 Helle, M J
A1 Krohn, K J
YR 1989
UL http://jcp.bmj.com/content/42/10/1043.abstract
AB Cytological imprint material from 26 mammary carcinomas was stained with monoclonal antibodies to oestrogen and progesterone receptors in an immunoperoxidase procedure. The staining result was compared with that of parallel stainings of frozen tissue sections of the same tumours. The peroxidase reactions in both techniques were semiquantitatively assessed (histoscore). In both sets of stainings the results agreed in 25 of 26 cases (oestrogen receptor: 19 positive, six negative; progesterone receptor: 14 positive, 11 negative). The histoscores of imprint preparations and cryostat sections showed a significant correlation in linear regression analysis (oestrogen receptor: r = 0.755, p less than 0.001; progesterone receptor: r = 0.740, p less than 0.001). Imprint cytology is simple, does not require expensive instruments, and no separate specimen has to be sequestered. It is especially suitable for immunocytochemical steroid receptor analysis of small breast carcinomas.