PT  - JOURNAL ARTICLE
AU  - Green, M A
AU  - Sviland, L
AU  - Malcolm, A J
AU  - Pearson, A D
TI  - Improved method for immunoperoxidase detection of membrane antigens in frozen sections.
AID  - 10.1136/jcp.42.8.875
DP  - 1989 Aug 01
TA  - Journal of Clinical Pathology
PG  - 875--880
VI  - 42
IP  - 8
4099  - http://jcp.bmj.com/content/42/8/875.short
4100  - http://jcp.bmj.com/content/42/8/875.full
SO  - J Clin Pathol1989 Aug 01; 42
AB  - The visualisation of membrane antigens in frozen sections using metal-enhanced 3,3'diaminobenzidine (DAB) was investigated. Particular attention was paid to the degree of reaction enhancement, the disruptive effect on morphological detail, and the ease of the techniques. The best result was obtained using nickel-modified DAB at pH 6.0 to develop the peroxidase enzyme, with further enhancement in cobalt chloride at neutral pH. Silver methenamine enhancement is also possible but can give rise to non-specific or background staining.