PT  - JOURNAL ARTICLE
AU  - Singh, R
AU  - Gorton, A W
TI  - Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium.
AID  - 10.1136/jcp.42.8.881
DP  - 1989 Aug 01
TA  - Journal of Clinical Pathology
PG  - 881--884
VI  - 42
IP  - 8
4099  - http://jcp.bmj.com/content/42/8/881.short
4100  - http://jcp.bmj.com/content/42/8/881.full
SO  - J Clin Pathol1989 Aug 01; 42
AB  - Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.