RT Journal Article
SR Electronic
T1 Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 881
OP 884
DO 10.1136/jcp.42.8.881
VO 42
IS 8
A1 Singh, R
A1 Gorton, A W
YR 1989
UL http://jcp.bmj.com/content/42/8/881.abstract
AB Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.