RT Journal Article
SR Electronic
T1 Use of immunocytochemistry and biotinylated in situ hybridisation for detecting measles virus in central nervous system tissue.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 329
OP 333
DO 10.1136/jcp.43.4.329
VO 43
IS 4
A1 McQuaid, S
A1 Isserte, S
A1 Allan, G M
A1 Taylor, M J
A1 Allen, I V
A1 Cosby, S L
YR 1990
UL http://jcp.bmj.com/content/43/4/329.abstract
AB Optimised immunocytochemical (ICC) and in situ hybridisation (ISH) protocols for long term, formalin fixed, central nervous system tissue infected with measles virus were developed. The effectiveness of 10 proteases for the enzymatic unmasking of formalin fixed antigen and nucleic acid was investigated. Protease VIII gave maximal signal generation with optimal tissue preservation and no background staining for both techniques. The use of a microwave oven as an additional pre-hybridisation step for RNA-RNA in situ hybridisation produced a significant increase in the number of cells labelled for genomic RNA. The ability to show the presence of antigen and nucleic acid in long term, formalin fixed tissue facilitates the use of stored necropsy material available in pathology departments for ICC and ISH investigations.