PT  - JOURNAL ARTICLE
AU  - Landers, R J
AU  - O'Leary, J J
AU  - Crowley, M
AU  - Healy, I
AU  - Annis, P
AU  - Burke, L
AU  - O'Brien, D
AU  - Hogan, J
AU  - Kealy, W F
AU  - Lewis, F A
TI  - Epstein-Barr virus in normal, pre-malignant, and malignant lesions of the uterine cervix.
AID  - 10.1136/jcp.46.10.931
DP  - 1993 Oct 01
TA  - Journal of Clinical Pathology
PG  - 931--935
VI  - 46
IP  - 10
4099  - http://jcp.bmj.com/content/46/10/931.short
4100  - http://jcp.bmj.com/content/46/10/931.full
SO  - J Clin Pathol1993 Oct 01; 46
AB  - AIM--To detect the presence or absence of Epstein-Barr virus (EBV) in cervical lesions ranging from normality to invasive malignancy. METHODS--Eighteen randomly selected cases of invasive squamous cell carcinomas of the uterine cervix were examined as well as 25 cases each of normal cervices and those showing cervical intra-epithelial neoplasia (CIN) I, II, and III. DNA-DNA in situ hybridisation, using a biotinylated probe to the Bam H1 "W" fragment of EBV, was carried out in addition to the polymerase chain reaction using specific primer sequences that flank a 153 base pair segment of the Bam H1 "W" region of the EBV genome and which do not cross-amplify other DNA herpes viruses. Positive control material included paraffin wax embedded P3 HR1 lymphoblastoid cells (containing high copy numbers of EBV) and two nasopharyngeal carcinomas positive for EBV. RESULTS--Neither normal nor CIN I tissue was positive. Eight per cent of CIN II tissue was positive; 8% of CIN III, and 43% of carcinomas were positive for EBV. CONCLUSION--The study shows that the virus is present in some cases of cervical carcinoma and to a lesser degree in some premalignant lesions of the cervix, but the exact association between it and cervical oncogenesis, be it causative or incidental, remains to be determined.