PT  - JOURNAL ARTICLE
AU  - Kerr, J R
AU  - Taylor, G W
AU  - Rutman, A
AU  - Høiby, N
AU  - Cole, P J
AU  - Wilson, R
TI  - Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth.
AID  - 10.1136/jcp.52.5.385
DP  - 1999 May 01
TA  - Journal of Clinical Pathology
PG  - 385--387
VI  - 52
IP  - 5
4099  - http://jcp.bmj.com/content/52/5/385.short
4100  - http://jcp.bmj.com/content/52/5/385.full
SO  - J Clin Pathol1999 May 01; 52
AB  - AIM: To examine strains of Pseudomonas aeruginosa for specific antifungal factors. METHODS: Two clinical strains of P aeruginosa with strong in vitro inhibition (by cross streak assay) of Candida albicans and Aspergillus fumigatus were examined. Both strains were isolated from sputum--one from a patient with cystic fibrosis and one from a patient with bronchiectasis. Bacterial extracts were fractionated by high performance liquid chromatography and examined by ultraviolet absorbance and mass spectroscopy. Antifungal activity against C albicans and A fumigatus was determined in a well plate assay. RESULTS: Pyocyanin was the major antifungal agent of P aeruginosa; 1-hydroxy-phenazine also possessed activity. Pyocyanin MICs for C albicans and A fumigatus were > 64 micrograms/ml. These phenazines were active against nine other yeast species pathogenic for man. Preliminary experiments also suggested possible inhibition of yeast mycelial transformation in C albicans by pyocyanin. CONCLUSIONS: There may be a role for pyocyanin and 1-hydroxyphenazine in the prevention of pulmonary candidiasis in patients colonised by P aeruginosa.