PT  - JOURNAL ARTICLE
AU  - Pietzsch, J
AU  - Bergmann, R
TI  - Measurement of 5-hydroxy-2-aminovaleric acid as a specific marker of metal catalysed oxidation of proline and arginine residues of low density lipoprotein apolipoprotein B-100 in human atherosclerotic lesions
AID  - 10.1136/jcp.56.8.622
DP  - 2003 Aug 01
TA  - Journal of Clinical Pathology
PG  - 622--623
VI  - 56
IP  - 8
4099  - http://jcp.bmj.com/content/56/8/622.short
4100  - http://jcp.bmj.com/content/56/8/622.full
SO  - J Clin Pathol2003 Aug 01; 56
AB  - γ-Glutamyl-semialdehyde (γGSA) is a major product of the metal catalysed oxidation of apolipoprotein B-100 (apoB-100) proline and arginine residues. On reduction, γGSA forms 5-hydroxy-2-aminovaleric acid (HAVA). This report describes the application of HAVA measurement to characterise the formation of γGSA in low density lipoprotein (LDL) recovered from human atherosclerotic lesions. HAVA concentrations were greatly increased in LDL from early (mean, 10.25; SD, 3.49 mol/mol apoB-100; p < 0.01), intermediate (mean, 11.18; SD, 2.37 mol/mol apoB-100; p < 0.01), and advanced (mean, 9.91; SD, 2.15 mol/mol apoB-100; p < 0.01) lesions, when compared with LDL from normal aortic tissue (mean, 0.05; SD, 0.01 mol/mol apoB-100). These findings support the hypothesis that pathways involving metal catalysed oxidation of LDL apoB-100 are of pathological importance in atherogenesis.