RT Journal Article
SR Electronic
T1 Expression of β-catenin in gastroenteropancreatic endocrine tumours: a study of 229 cases
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1300
OP 1304
DO 10.1136/jcp.2005.035097
VO 59
IS 12
A1 Hervieu, V
A1 Lepinasse, F
A1 Gouysse, G
A1 Guillaud, O
A1 Barel, C
A1 Chambonniere, M-L
A1 Bringuier, P-P
A1 Poncet, G
A1 Lombard-Bohas, C
A1 Partensky, C
A1 Chayvialle, J-A
A1 Scoazec, J-Y
YR 2006
UL http://jcp.bmj.com/content/59/12/1300.abstract
AB Aims: To clarify the role of β-catenin in digestive endocrine carcinogenesis, a large and representative series of gastroenteropancreatic endocrine tumours was analysed in order to determine the incidence and pattern of β-catenin changes and to analyse the clinical and histological characteristics of the tumours presenting immunohistochemically detectable changes in β-catenin expression. Methods: 229 cases of gastroenteropancreatic endocrine tumours (stomach, 11; duodenum and ampulla, 29; jejunum and ileum, 51; appendix, 13; colon and rectum, 17; and pancreas, 108) were studied by immunohistochemistry to assess the pattern of distribution of β-catenin (membranous, cytoplasmic or nuclear). DNA was analysed to detect mutations in exon 3 of the CTNNB1 gene. Results: The distribution of immunoreactive β-catenin protein was membranous in 164 cases, cytoplasmic in 58 cases and nuclear in seven cases. No mutation was detected in exon 3 of the CTNNB1 gene in any case. The seven cases with nuclear accumulation of β-catenin were large tumours (mean size 44 (standard deviation (SD) 18.5) mm) with metastases, including liver metastases in five cases, high Ki-67 index (mean 34% (SD 16.5%)) and cyclin D1 overexpression; p53 accumulation was detected in six cases. Five patients died of disease; the mean (SD) survival was 13.6 (4.8) months. Conclusions: Immunohistochemically detectable nuclear accumulation of β-catenin is infrequent in gastroenteropancreatic endocrine tumours and is usually not associated with mutations in CNNTB1 exon 3. Changes in β-catenin expression are late events in digestive endocrine carcinogenesis, associated with tumour progression and dissemination.