RT Journal Article
SR Electronic
T1 Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 903
OP 911
DO 10.1136/jcp.2004.020610
VO 59
IS 9
A1 Naresh, K N
A1 Lampert, I
A1 Hasserjian, R
A1 Lykidis, D
A1 Elderfield, K
A1 Horncastle, D
A1 Smith, N
A1 Murray-Brown, W
A1 Stamp, G W
YR 2006
UL http://jcp.bmj.com/content/59/9/903.abstract
AB Specimens of bone marrow trephine biopsy (BMT) are transported and fixed in acetic acid–zinc–formalin fixative, decalcified in 10% formic acid–5% formaldehyde and processed with other specimens to paraffin-wax embedding. Sections, 1-μm-thick, are cut by experienced histotechnologists and used for haematoxylin and eosin, Giemsa, reticulin silver and other histological stains. Further, all immunohistochemical procedures used in the laboratory, including double immunostaining, can be used on these sections with no or minimal modifications. About 10 000 BMT specimens have been analysed using this procedure since 1997 and diseases involving the bone marrow have been classified successfully. More recently, standardised polymerase chain reaction-based analysis and mRNA in situ hybridisation studies have been conducted. Excellent morphology with good antigen, DNA and RNA preservation is offered by the Hammersmith Protocol.