PT  - JOURNAL ARTICLE
AU  - Al-Shieban, Saeed
AU  - Tadrous, Paul J
AU  - Naresh, Kikkeri N
TI  - Estimation of cell density to aid in assessment of percentage cells of a particular lineage or of cells expressing a specific antigen in bone marrow trephine biopsies
AID  - 10.1136/jclinpath-2011-200126
DP  - 2013 Feb 01
TA  - Journal of Clinical Pathology
PG  - 155--159
VI  - 66
IP  - 2
4099  - http://jcp.bmj.com/content/66/2/155.short
4100  - http://jcp.bmj.com/content/66/2/155.full
SO  - J Clin Pathol2013 Feb 01; 66
AB  - The authors aimed to develop a tool to assess total cell numbers in a microscope's field of vision, which would provide the denominator for calculating the percentage of positive cells for a given antigen in bone marrow trephine biopsies (BMTBs) of varying cellularities. Precise estimates of cell densities were made from 179 images of BMTBs of varying cellularities using a cell-counting software. The estimates were then validated on an independent set of 20 BMTBs. Among the 179 images, there was a strong linear association between marrow cellularity and cell numbers (Pearson correlation: 0.788). Then standardised cell densities (cells/mm2 of bone marrow) were deduced for BMTBs of varying cellularities. In the validation study, the actual and the estimated cell numbers correlated strongly (Pearson correlation: 0.990). The cell density estimates provided in this study can be adapted for any microscope and the same method can be used for calculation of the percentage-positive cells for any antigen.