RT Journal Article
SR Electronic
T1 Rapid microdissection of tissue sections via laser ablation
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 430
OP 434
DO 10.1136/jcp-2023-209361
VO 77
IS 6
A1 Coope, Robin JN
A1 Pleasance, Stephen
A1 Pandoh, Pawan
A1 Schlosser, Colin
A1 Corbett, Richard D
A1 Marra, Marco A
YR 2024
UL http://jcp.bmj.com/content/77/6/430.abstract
AB We demonstrate a method for tissue microdissection using scanning laser ablation that is approximately two orders of magnitude faster than conventional laser capture microdissection. Our novel approach uses scanning laser optics and a slide coating under the tissue that can be excited by the laser to selectively eject regions of tissue for further processing. Tissue was dissected at 0.117 s/mm2 without reduction in yield, sequencing insert size or base quality compared with undissected tissue. From eight cases, 58–416 mm2 of tissue was obtained from one to four slides in 7–48 seconds total dissection time per case. These samples underwent exome sequencing and we found the variant allelic fraction increased in regions enriched for tumour as expected. This suggests that our ablation technique may be useful as a tool in both clinical and research labs.