Distribution of the available stains and final diagnosis within the case cohort retrieved, divided per scanner group
| Aperio CS2/FL | 3DHISTECH MIDI II | Hamamatsu NanoZoomer S60 | Total | |
| No of cases (n) | 60 | 60 | 60 | 180 |
| Histochemistry (n) | ||||
| H&E | 112 | 102 | 77 | 291 |
| PAS | 66 | 81 | 66 | 213 |
| Trichrome | 66 | 75 | 68 | 209 |
| Jones | 68 | 67 | 60 | 195 |
| Congo red | 5 | 12 | 13 | 30 |
| IF (n) | ||||
| IgG | 66 | 68 | 60 | 194 |
| IgA | 62 | 69 | 61 | 192 |
| IgM | 60 | 60 | 60 | 180 |
| C3 | 62 | 63 | 60 | 185 |
| C1q | 61 | 65 | 60 | 186 |
| Kappa | 63 | 72 | 60 | 195 |
| Lambda | 63 | 72 | 60 | 195 |
| IHC (n) | ||||
| PLA2R | 7 | 9 | 5 | 21 |
| THSD7A | 7 | 9 | 5 | 21 |
| DNAJB9 | 2 | 1 | 6 | 9 |
| Disease (n, %) | ||||
| MCD | 8 (13) | 8 (13) | 6 (10) | 22 (12) |
| MN | 7 (12) | 9 (15) | 5 (8) | 21 (12) |
| FSGS | 7 (12) | 6 (10) | 7 (12) | 20 (11) |
| IgA nephropathy | 2 (3) | 6 (10) | 11 (18) | 19 (11) |
| ANCA | 9 (15) | 4 (6) | 5 (8) | 18 (10) |
| DN | 5 (8) | 7 (12) | 6 (10) | 18 (10) |
| TIN | 6 (10) | 6 (10) | 5 (8) | 17 (9) |
| MGRS | 7 (12) | 4 (6) | 5 (8) | 16 (9) |
| Other | 9 (15) | 10 (16) | 10 (16) | 29 (16) |
ANCA, antineutrophilic cytoplasmic antibody; DN, diabetic nephropathy; DNAJB9, Dnaj (hsp40) homologue, subfamily b, member 9; FSGS, focal segmental glomerulosclerosis; IF, immunofluorescence; IHC, immunohistochemistry; MCD, minimal change disease; MGRS, monoclonal gammopathy of renal significance; MN, membranous nephropathy; PAS, periodic acid–Schiff; PLA2R, phospholipase A2 receptor; THSD7A, thrombospondin type 1 containing 7A domain; TIN, tubulointerstitial nephritis;